Colorimetric sensors are widely used for the detection of biomarkers related to conditions like diabetes, Parkinson’s disorder, etc. Protein biomarkers were used at low concentration due to which it showed difficulty in estimation. Henceforth, various attempts are made to increase the sensitivity of colorimetric sensor by using different amplification signals. Enzyme-based amplification signals are mostly differentiated for bioassay using ELISA (enzyme-linked immunosorbent assay).